Photographer: onbekend

dr. ing. F.L.W. (Frank) Takken


  • Faculty of Science
    SILS
  • Visiting address
    Science Park A
    Science Park 904  Room number: C2.214
  • Postal address:
    Postbus  94215
    1090 GE  Amsterdam
  • F.L.W.Takken@uva.nl
    T: 0205257795

Research interest

 

Unravelling the molecular and biochemical mechanism underlying disease resistance in plants.

My main aim is to understand, in molecular detail, how plants recognise pathogens and mount an effective defence response. Thereto, three research lines are pursued in my group. The eldest line focuses on function of NLR immune receptors in disease resistance. Earlier, we found that these proteins act as nucleotide-operated molecular switches, whose conformation changes upon pathogen perception (Fig 1 and i.e. Takken and Tameling, Science 2009, Takken and Goverse, COPB 2012). Recently we reported, in collaboration with the Cann laboratory (Durham UK), that the NB-ARC domain (Fig 2) NLR proteins can bind and bend DNA in vivo, and that DNA binding in planta requires proper activation by the genuine pathogen (Fenyk et al, JBC 2015). This exciting finding defines DNA as the first conserved molecular target of an NLR protein and in a recently funded VICI project the role of DNA binding by NLR proteins for plant immunity is chased.

Fig 1. Model for the switch function of NBS-LRR proteins. In the absence of a pathogen an NB-LRR protein resides in its resting (ADP) state, in which the LRR stabilizes the closed conformation. Recognition of the effector (here PVX-CP) is conferred by the C-terminal part of the LRR together with CC/TIR domain (CC). Perception of the effector (direct or indirect) changes the interaction surface between the N-terminal part of the LRR and the ARC2 subdomain, thereby releasing the autoinhibition conferred by the LRR. Subsequent nucleotide exchange triggers a second conformational change, altering the interactions of the NB-ARC domain with CC and LRR domains (induced state). In the activated state the NB subdomain is accessible to interact with DNA and other signaling partners. Hydrolysis of ATP could return the protein to its resting state (figure adapted from Takken and Tameling, Science 2009) and update with recent data published in Fenyk et al, 2015 JBC.

Fig 2. 3D structure of the NB-ARC domain Computationally derived 3D structure model of the NB-ARC domain of the resistance protein I-2. The model was created using the ADP bound structure of human APAF-1 (PDB code 1z6t, chain A) as  template. Locations of R protein specific motifs are marked with arrows. Amino acids of the MHD motif as well as the sensor I arginine are shown in stick representation. ADP atoms are depicted as balls-and-sticks. Subdomain coloring is: NB: red, ARC1: green, ARC2: blue. Atom coloring is: oxygen: red, nitrogen: blue, phosphorus: orange. (Figure adapted from van Ooijen et al, (2008) J. Exp. Bot, 59 (6):1383-1397)

Disease resistance can also be conferred by a lack of compatibility with a given pathogen (van Schie and Takken, Ann. Rev. Phytopathol 2015). In the second research line we focus on the identification of compatibility/susceptibility genes by screening for host protein targets that are manipulated by a pathogen. Our main model is the interaction between tomato and the soil-borne fungal pathogen  Fusarium oxysporum fsp.  lycopersici. Fusarium colonizes the xylem vessels of a plant causing wilt disease. During infection the fungus secretes many small proteins in the xylem sap that are referred to as Six (Secreted In Xylem) proteins. Many Six proteins promote disease development, like Six6 (Gawehns et al MPMI 2014) or Six3 (Avr2) (Houterman et al 2009 Plant J) that acts in the nucleus (Ma et al Front Plant Sci 2013) in conjunction with Six5 (Ma et al New Phytol 2015). Besides enhancing virulence Avr2 is also triggering an I-2 specific immune response (Fig 3). Using Six proteins as probes we have successfully identified specific plant proteins whose knockout result in increased disease resistance. Identification of these susceptibility genes increases our understanding on how a pathogen manipulates its host providing new leads to combat diseases (Gawehns et al, Micr. Biotech 2013).

In my 3  rd and newest research line we focus on the intriguing observation that colonisation by endophytic fungi can enhance disease resistance to biotic and abiotic stresses. In this project we study a) how endophytes evade host resistance while at the same time enhance immunity and b) how endophytes differ from pathogens, being both able to overcome defences and colonise the host, yet with a different outcome for the latter.

Fig 3. Agroinfiltration in a Nicotiana benthamiana leaf of I-2 (upper circles) and Avr2 (lower circles) triggers a hypersensitive cell death response only where both proteins are present. No cell death is observed on the left side where an Avr2 variant is expressed that is not recognized by I-2 (Houterman et al, Plant J 2009)

Plant pathology group

The Plant Pathology group is headed by Prof.dr. Ben Cornelissen. The group is divided into three subgroups: my "plant" group, the "fungus" group and the "Sumo" group, the latter groups are headed by respectively dr. Martijn Rep and Dr. Harrold van den Burg.

Prof Marcel Prins is appointed a special chair Virology in the Plant Pathology group and in close collaboration these groups study the molecular interaction between plants and their (fungal) pathogens. 

People involved:

 

Dr Marijn Knip

   Postdoc - VICI project: Linking plant immunity and DNA damage. 

 

Dr Manon Richard

   Postdoc - VICI project: Linking DNA binding of plant NLR proteins to plant immunity.

 

Dr Nico Tintor

   Postdoc - Collaboration ENZA/MPP: The role of effector uptake in plant immunity. 

 

Mila Blekemolen

   PhD student - VICI project: Elucidating the molecular mechanism underlying the virulence and avirulence functions of the Fusarium Avr2/Six5 effector pair.

 

Machiel Beijaert

   Technician - VICI project: supporting the VICI project in various ways.

 

Francisco de Lamo Ruiz (shared supervision with Dr M Rep)

   PhD student - ETN Horizon2020 : How do endophytes evade host resistance while enhancing immunity?

 

Maria Constantin  (shared supervision with Dr M Rep)

   PhD student - ETN Horizon202: “How do endophytes differ from pathogens?” 

 

Former lab members

Lingxue Cao
   PhD student: Elucidating the molecular mechanism underlying the virulence and avirulence functions of the Fusarium Avr2/Six5 effector pair

Xiaotang Di
    PhD student: The Avr2 effector protein from Fusarium as a guide to unravel plant innate immunity.

Dirk-Jan Valkenburg
 
 Technician: involved in all ongoing projects in the group.

Dr Hanna Richter
 
 Technician: involved in all ongoing projects in the group.

ir. Lisong Ma
    PhD student: Analysing the intrinsic functions of Avr2 and its perceival by the resistance protein I-2.  

Fleur Gawehns
   PhD student: Functional characterisation of Fusarium secreted effector proteins in disease and resistance

ir. Ewa Lukasik
     PhD student: focussing on the ATPase activity of the NBS domain of R proteins and the identification of interacting protein
drs Vladimir Krasikov
    Postdoc analyzing the role of the Xsp10 protein for resistance anddisease to Fusarium oxysporum
dr. ir. Harrold van den Burg
    Veni Postdoc studying the role of SUMO modification in plant defence signalling
ing Marianne de Vroomen
   Technician, involved in most ongoing projects.
Drs Mobien Kasiem
  PhD student: PhD student: Structure and function of tomato disease resistance proteins
Dr Gerben van Ooijen
  PhD student: Structure and function of tomato disease resistance proteins
Dr Klaas Jan de Vries
  Postdoc:  Targeted proteomics of signalosomes mediating pathogen resistance
Dr Wladimir Tameling
  PhD student: Disease resistance proteins of the NBS-LRR class, molecular switches of plant defence
Dr Sergio de la Fuente van Bentem
   PhD student: Unfolding plant disease resistance, the involvement of HSP90 and its co-chaperone  PP5 in I-2 mediated signalling
Dr Jack Vossen
  Postdoc: Characterisation of the I-2 signalosome
Dr Sandra Elzinga
   Postdoc: Characterisation of the Mi-1 signalosome

Invited speaker

Selection from the last five years

  • The Fusarium oxysporum effector Six8 manipulates plant immunity through association with the transcriptional co-repressors TPL and TPR1. 28 August 2017,  Shanghai Center for Plant Stress Biology, Shanghai,  P.R.  China.
  • The Fusarium oxysporum Avr2-Six5 effector pair alters the exclusion selectivity of plasmodesmata and targets PTI signaling. 23 August 2017. Nanjing Agricultural University, Lab of Molecular Plant Virology, Nanjing. P.R.  China.
  • The Fusarium oxysporum Avr2-Six5 effector pair alters exclusion selectivity of plasmodesmata, 12 July 2017, EMBO Workshop; Intracellular Communication in development and disease, Harnack House, Berlin, Germany 
  • Functional analysis of the Fusarium Avr2 effector protein. 24 March 2017, Gregor Mendel Insitute Vienna Austria.
  • The role of Fusarium effectors in virulence and NLR-mediated innate immunity. December 1 2016, Institut für Biologie/Genetik Martin-Luther-Universität,  Halle (Saale), Germany
  • Functional analysis of the Fusarium Avr2 effector protein. October 26  016, Sainsbury Laboratory, Norwich United Kingdom
  • Gene-for-gene resistance - old dogma’s new insights: NLR’s, sentinels of plant immunity. TSL Summer School: Plant Microbe Interactions. 17-28 August 2015. TSL Norwich, United Kingdom
  • The Fusarium oxysporum effector Six8 manipulates plant immunity through association with the transcriptional co-repressors TPL and TPR1. Annual Conference of the SUSTAIN COST Action (FA1208)   17-19 February 2016 Banyuls, France 
  • The role of Fusarium effectors in virulence and NLR-mediated innate immunity.  New Phytologist workshop; “The apoplast as battleground for plant-microbe interactions”. 9 th– 10 st July 2015, Castle Rauischholzhausen, Gießen, Germany
  • The Fusarium oxysporum effector Six8 manipulates plant immunity through association with the transcriptional co-repressors TPL and TPR1. International Conference on “Plant Biotic Stresses & Resistance Mechanisms II” July 2 - 4, 2015 Vienna, Austria
  • The NLR immune receptors I-2 and Rx1 are DNA deforming proteins. 13-14 April 2015 De Wereld, Lunteren, The Netherlands
  • The NLR immune receptors I-2 and Rx1 are DNA deforming proteins. MPI symposium ‘NLR Biology in Plants and Animals’, May 3-6, 2015 Schloss Ringberg Munich, Germany
  • The NLR immune receptors I-2 and Rx1 are DNA deforming proteins. 15 Jan 2015 University of Copenhagen Copenhagen, Denmark
  • The Fusarium oxysporum effector Six8 manipulates plant immunity through association with the transcriptional co-repressors TPL and TPR1. MINI-SYMPOSIUM: Wageningen University 24 Nov 2014, Wageningen Campus - Netherlands
  • The tomato-fusarium pathosystem: a game of hide and seek, 16th IS-MPMI July 2014 Rhodos Greece
  • The Fusarium oxysporum effector Six8 manipulates plant immunity through association with the transcriptional co-repressors TPL and TPR1. COST WORKSHOP: Structure-guided investigation of effector function, action and recognition. 10 – 12 Sept 2014, Bucharest, Romania
  • De wapenwedloop tussen planten en hun belagers, een strijd om leven en dood.  5 March 2014  Spui lezing Amsterdam
  • The role of Fusarium effectors in NLR-mediated innate immunity. XV International Congress on Molecular Plant-Microbe Interactions (IS-MPMI) July 29-August 2, 2012, Kyoto, Japan
  • How to build a pathogen detector; structural basis of NB-LRR function. EMBO practical course 18-29 june 2012 TSL, Norwich, UK
  • The role of Fusarium effectors in NLR-mediated plant innate immunity. 9 April 2012, Plant Biology Department, University of Fribourg, Fribourg Switzerland.
  • The role of pathogen effectors in NLR-mediated plant innate immunity. International Meeting of the Collaborative Research Center SFB 648  "Communication in Plants and their Responsesto the Environment" May 19-22, 2011, Halle (Saale), Germany 
  • Molecular co-evolution in the tomato-Fusarium pathosystem. October 8, 2010, Justus Liebig University , Giessen, Germany
  • Functional analysis of Fusarium oxysporum f.sp. lycopersici effector proteins. DFG funded colloquium: 'Microbial reprogramming of plant cell development' priority project SPP1212 'Plant-Micro' June 2010,  Freising, Germany
  • The arms race between effectors of Fusarium oxysporum and resistance proteins of tomato. Seminar series School of Biological and Biomedical Sciences, May 2010. Durham University, Durham, UK
  • How to resist a resistance protein? British Society for Plant Pathology, Presidential Meeting, 2009, Oxford, UK
  • How to resist tomato resistance proteins? XIV International Congress on Molecular Plant-Microbe Interactions. July 19-23, 2009, Quebec, Canada.
  • Resistance proteins: scouts of the plant innateimmune system. 18th EUCARPIA congress, "Modern Variety Breeding for Present and Future Needs" 9-12 September 2008, Valencia, Spain
  • Structure-function analysis of plant NB-LRR disease resistance proteins. SFB 670 " Structure Function and Evolution of Innate immunity" 22-23 September 2008, Max-Planck-Institut für Züchtungsforschung, Koln, Germany
  • Resistance proteins: scouts of the plant innate immune system. EPS/Bioexploit Summerschool "On the evolution of plant-pathogen interactions: from principles to practices" 18-20 June 2008, Wageningen, the Netherlands.
  • Resistance proteins: scouts of the plant innate immune system.  4th EPSO Conference, 22-26 June 2008 , Presqu'ile de Giens (Côte d'Azur), France
  •  Molecular aspects of I-2 mediated resistanceto Fusarium oxysporum . Seminar series. May 2008, Max-Planck-Institut für Züchtungsforschung, Koln, Germany
  • Function of the Nucleotide Binding Domain for R Proteins. Keystone Symposium on Plant Innate Immunity, Feb 2008 Keystone Resort,  Keystone, Colorado, USA
  • The NB-ARC domain of R proteins acts asa molecular switch regulating plant innate immunity.  (2007) Max von Pettenkofer Institut, LMU München, Germany
  • Host Pathogen interactions from Plants to Mammals - Distinct and shared pathways of immune defense. Inflammatory Diseases of Barrier Organs 2007-Genetic exploration leads to novel therapies in inflammation, Kiel, Germany.
  • The NB-ARC domain: an NTP-hydrolysing molecular switch. Banbury meeting: Comparative Biology of Innate Immune Systems, (2006) New York, USA
  • Innate immunereceptors in plants and animals; do analogous modules have comparable functions? EPS Symposium Intracellular signalling, (2006)Amsterdam, UvA
  • R proteins, molecular switches of plant defence, 8th Conference of the European Foundation for Plant Pathology & British Society of Plant Pathology Presidential Meeting, 2006 Copenhagen, Denmark

Publications arising from my PhD and Postdoc period, for recent papers see Tab "publications"

Papers <2002

  • C. F. de Jong, F. L. W. Takken , X. Cai, P. J. G. M. de Wit and M. H. A.J. Joosten (2002)Attenuation of Cf -mediated defenseresponses atelevated temperatures correlates withadecrease in elicitor-binding sites.Molecular Plant-Microbe Interactions 15: 10. 1040-9.
  • P. J. G. M. de Wit, B. F. Brandwagt, H. A. Van den Burg, X. Cai, R. A. L. Van der Hoorn, C.F. De Jong, J. van 't Klooster, M. J. D. de Kock, M. Kruijt, W. H. Lindhout, R. Luderer, F. L. W. Takken , N. Westerink, J.J. M. vervoort and M. H. A. J. Joosten (2002) The molecular basis of co-evolution between Cladosporium fulvum  and tomato. Antonie vanLeeuwenhoek 81: 409-412
  • R. Luderer, F. L. W. Takken , P.J. G.m.Witand M. H. A. J. Joosten (2002) Cladosporiumfulvum overcomes Cf-2 -mediated resistance by producing truncated AVR2 elicitor proteins. Molecular Microbiology 45: 3. 875-84.
  • W. I. L. Tameling, S.D. Elzinga,P.S. Darmin, J. H. Vossen, F. L. W. Takken , M.A.Haring and B. J. C. Cornelissen (2002) The tomato R gene products I-2 and Mi-1 are functional ATP binding proteins withATPase activity. Plant Cell 14: 11. 2929-39.
  • X. Cai, F. L. W. Takken , M. H. A. J. Joosten and P.J. G. M. De Wit(2001) Specific recognition of Avr4 and Avr9 result in distinct patternsof hypersensitive cell death in tomato, but similar patterns ofdefence-related geneepxression. Molecular Plant Pathology 2: 2. 77-86
  • B. F. Brandwagt, L. A.Mesbah, F. L. W. Takken , P. L. Laurent, T. J. A. Kneppers, J. Hille and H. J. J. Nijkamp (2000) A longevity assurance gene homolog of tomatomediates resistanceto Alternaria alternata f. sp. lycopersici toxins and fumonisin B1. Proc Natl Acad Sci USA 97:9.4961-6.
  • F.L. W.Takken and M. H. A. J.Joosten (2000) Plant resistance genes: their structure, function and evolution. European Journal of Plant Pathology 106: 8. 699-713
  • F. L. W. Takken, R. Luderer, S. H. E. J. Gabriëls, N.Westerink, R. Lu, P.J.G. M. de Wit and M.H.A.J.Joosten (2000) A functional cloning strategy, based on a binaryPVX-expression vector, toisolate HR-inducing cDNAsof plant pathogens. The Plant Journal 24: 2). 275-283
  • L. A.Mesbah, T.J. A. Kneppers, F. L. W. Takken, P. Laurent,J.Hille and H. J. J. Nijkamp (1999) Geneticand physical analysis of aYAC contig spanning the fungal diseaseresistancelocus Asc of tomato ( Lycopersicon esculentum ). Molecular and General Genetics 261 (1): 50-57
  • F.L. W. Takken , M. Thomas, M.H. A. J. Joosten, C. Golstein, N.Westerink, J. Hille, H. J. J. Nijkamp, P. J. G. M. DeWit and J. D. G. Jones (1999) A second gene at the tomato Cf-4 locus confers resistance to Cladosporium fulvum through recognition of anovel avirulence determinant. The Plant Journal 20: 3. 279-288
  • F.L. W. Takken , D. Schipper, H. J. J. Nijkamp and J.Hille (1998)Identification and Ds-tagged isolation of a new gene at the Cf-4 locus of tomato involved in disease resistance to Cladosporium fulvum race 5. The Plant Journal 14: 401-411

patents

  • Takken, F.L.W., &Wit, P.J.G.M.de (10-01-2002). Elicitor from Cladosporium . no WO02/02787.
  • Turk, S.C.H.J., Takken, F.L.W., Jong, C.F. de, Joosten, M.H.A.J.,& Wit, P.J.G.M. de (02-01-2003). Nucleotide sequences involved in plantdisease resistance.no01202420.4./ WO03000930  
  • Joosten, M.H.A.J., Jong, C.F. de, Wit, P.J.G.M. de, Takken, F.L.W., & Turk, S.C.H.J. (17-03-2004). Nucleotide sequences involved in plantresistance. no EP1397515

Biography

Frank Takken (1969) is interested in how plants defend themselves against pathogens. Plants are able to sense the presence of invading microbes. Recognition subsequently results in activation of the plant innate immune system. In this process, resistance proteins play a key role as they mediate pathogen recognition and trigger the activation of downstream signaling cascades that halt the pathogen and thereby prevent disease. An example of a succesfull immune response is shown in the figure below where the tomato  Immunity-2 ( I-2) gene halts ingress of the pathogenic fungus Fusarium oxysporum.

Resistance genes come in different flavors, but the majority encodes intracellular tri-partite proteins that contain a central nucleotide-binding domain. One of Frank's major research interests is the role the distinct domains in these proteins have for their function. Structure-function analysis in combination with 3D modeling of the different subdomain is used to predict mutations that will have specific effects on the activity of these proteins. These mutants are subsequently analyzed for altered biochemical properties, differences in inter- and intramolecular interactions as well as for their effect on disease resistance.

To examine how resistance proteins activate downstream signaling it is essential to know their interacting partners. Using various screens, candidate interacting proteins, and recently also DNA, have been identified and these interactors are analyzed for their involvement in plant defense.

Frank is appointed as Associate professor in the Molecular Plant Pathology group at the Swammerdam Institute for Life Sciences and is since 2012 Scientific Advisor for Scienza Biotechnology. Earlier he

worked as postdoc at the Phytopathology group at Wageningen University. During this time he worked on a self-written project at the biotech company Keygene NV in Wageningen. In that project he focused on the identification of genes that are transcriptionally regulated during the onset of plant defense signaling. He received his PhD in 1999 from the Free University of Amsterdam for his pioneering work on the isolation of resistance genes from tomato.

A resistant tomato plant carrying I-2 (left) and susceptible one (right side) infected with Fusarium oxysporum f.sp. lycopersici.

2017

2016

  • Di, X., Gomila, J., Ma, L., van den Burg, H. A., & Takken, F. L. W. (2016). Uptake of the Fusarium Effector Avr2 by Tomato Is Not a Cell Autonomous Event. Frontiers in Plant Science, 7, [1915]. DOI: 10.3389/fpls.2016.01915  [details] 
  • Di, X., Takken, F. L. W., & Tintor, N. (2016). How Phytohormones Shape Interactions between Plants and the Soil-Borne Fungus Fusarium oxysporum. Frontiers in Plant Science, 7, [170]. DOI: 10.3389/fpls.2016.00170  [details] 
  • Fenyk, S., Dixon, C. H., Gittens, W. H., Townsend, P. D., Sharples, G. J., Pålsson, L. O., ... Cann, M. J. (2016). The Tomato Nucleotide-binding Leucine-rich Repeat (NLR) Immune Receptor I-2 Couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange. The Journal of Biological Chemistry, 291(3), 1137-1147. DOI: 10.1074/jbc.M115.698589  [details] 
  • Peng, H. C., Mantelin, S., Hicks, G. R., Takken, F. L. W., & Kaloshian, I. (2016). The Conformation of the Plasma Membrane-Localized SlSERK1 - Mi-1.2 Complex is Altered by a Potato Aphid Derived Effector. Plant Physiology, 171(3), 2211-2222. DOI: 10.1104/pp.16.00295  [details] 

2015

2014

  • Gawehns, F., Houterman, P. M., Ait Ichou, F., Michielse, C. B., Hijdra, M., Cornelissen, B. J. C., ... Takken, F. (2014). The Fusarium oxysporum effector Six6 contributes to virulence and suppresses I-2 mediated cell death. Molecular Plant-Microbe Interactions, 27(4), 336-348. DOI: 10.1094/MPMI-11-13-0330-R  [details] 
  • van Schie, C. C. N., & Takken, F. L. W. (2014). Susceptibility genes 101: how to be a good host. Annual Review of Phytopathology, 52, 551-581. DOI: 10.1146/annurev-phyto-102313-045854  [details] 

2013

2012

  • Lukasik-Shreepaathy, E., Slootweg, E., Richter, H., Goverse, A., Cornelissen, B. J. C., & Takken, F. L. W. (2012). Dual regulatory roles of the extended N terminus for activation of the tomato MI-1.2 resistance protein. Molecular Plant-Microbe Interactions, 25(8), 1045-1057. DOI: 10.1094/MPMI-11-11-0302  [details] 
  • Ma, L., Lukasik, E., Gawehns, F., & Takken, F. L. W. (2012). The use of agroinfiltration for transient expression of plant resistance and fungal effector proteins in Nicotiana benthamiana leaves. In M. D. Bolton, & B. P. H. J. Thomma (Eds.), Plant fungal pathogens: methods and protocols (pp. 61-74). (Methods in molecular biology; No. 835). New York: Humana Press. DOI: 10.1007/978-1-61779-501-5_4  [details] 
  • Takken, F. L. W., & Goverse, A. (2012). How to build a pathogen detector: structural basis of NB-LRR function. Current Opinion in Plant Biology, 15(4), 375-384. DOI: 10.1016/j.pbi.2012.05.001  [details] 
  • Bai, S., Liu, J., Chang, C., Zhang, L., Maekawa, T., Wang, Q., ... Shen, Q. H. (2012). Structure-function analysis of barley NLR immune receptor MLA10 reveals its cell compartment specific activity in cell death and disease resistance. PLoS Pathogens, 8(6), [e10027]. DOI: 10.1371/journal.ppat.1002752  [details] 
  • Lukasik-Shreepaathy, E., Vossen, J. H., Tameling, W. I. L., de Vroomen, M. J., Cornelissen, B. J. C., & Takken, F. L. W. (2012). Protein-protein interactions as a proxy to monitor conformational changes and activation states of the tomato resistance protein I-2. Journal of Experimental Botany, 63(8), 3047-3060. DOI: 10.1093/jxb/ers021  [details] 

2011

  • Krasikov, V., Dekker, H. L., Rep, M., & Takken, F. L. W. (2011). The tomato xylem sap protein XSP10 is required for full susceptibility to Fusarium wilt disease. Journal of Experimental Botany, 62(3), 963-973. DOI: 10.1093/jxb/erq327  [details] 
  • Maekawa, T., Cheng, W., Spiridon, L. N., Töller, A., Lukasik, E., Saijo, Y., ... Schulze-Lefert, P. (2011). Coiled-coil domain-dependent homodimerization of intracellular barley immune receptors defines a minimal functional module for triggering cell death. CELL Host & Microbe, 9(3), 187-199. DOI: 10.1016/j.chom.2011.02.008  [details] 
  • Mantelin, S., Peng, H. C., Li, B., Atamian, H. S., Takken, F. L. W., & Kaloshian, I. (2011). The receptor-like kinase SlSERK1 is required for Mi-1-mediated resistance to potato aphids in tomato. Plant Journal, 67(3), 459-471. DOI: 10.1111/j.1365-313X.2011.04609.x  [details] 

2010

  • Ament, K., Krasikov, V., Allmann, S., Rep, M., Takken, F. L. W., & Schuurink, R. C. (2010). Methyl salicylate production in tomato affects biotic interactions. Plant Journal, 62(1), 124-134. DOI: 10.1111/j.1365-313X.2010.04132.x  [details] 
  • Takken, F., & Rep, M. (2010). The arms race between tomato and Fusarium oxysporum. Molecular Plant Pathology, 11(2), 309-314. DOI: 10.1111/j.1364-3703.2009.00605.x  [details] 
  • Takken, F., van Ooijen, G., Lukasik, E., Ma, L., Gawehns, F., Houterman, P., & Rep, M. (2010). How to resist a tomato resistance gene? In H. Antoun, T. Avis, L. Brisson, D. Prévost, & M. Trepanier (Eds.), Proceedings of the 14th International Congress on Molecular Plant-Microbe Interactions: Quebec City, Quebec, Canada, July 19-23, 2009 (Biology of plant-microbe interactions; Vol. 7). St. Paul, MN: International Society for Molecular Plant-Microbe Interactions. [details] 
  • van Ooijen, G., Lukasik, E., van den Burg, H. A., Vossen, J. H., Cornelissen, B. J. C., & Takken, F. L. W. (2010). The small heat shock protein 20 RSI2 interacts with and is required for stability and function of tomato resistance protein I-2. Plant Journal, 63(4), 563-572. DOI: 10.1111/j.1365-313X.2010.04260.x  [details] 
  • van den Burg, H. A., Kini, R. K., Schuurink, R. C., & Takken, F. L. W. (2010). Arabidopsis small ubiquitin-like modifier paralogs have distinct functions in development and defense. The Plant Cell, 22(6), 1998-2016. DOI: 10.1105/tpc.109.070961  [details] 

2009

  • Houterman, P. M., Ma, L., van Ooijen, G., de Vroomen, M. J., Cornelissen, B. J. C., Takken, F. L. W., & Rep, M. (2009). The effector protein Avr2 of the xylem-colonizing fungus Fusarium oxysporum activates the tomato resistance protein I-2 intracellularly. Plant Journal, 58(6), 970-978. DOI: 10.1111/j.1365-313X.2009.03838.x  [details] 
  • Lukasik, E., & Takken, F. L. W. (2009). STANDing strong, resistance proteins instigators of plant defence. Current Opinion in Plant Biology, 12(4), 427-436. DOI: 10.1016/j.pbi.2009.03.001  [details] 
  • Takken, F. L. W., & Tameling, W. I. L. (2009). To nibble at plant resistance proteins. Science, 324(5928), 744-746. DOI: 10.1126/science.1171666  [details] 
  • van den Burg, H. A., & Takken, F. L. W. (2009). Does chromatin remodeling mark systemic acquired resistance? Trends in Plant Science, 14(5), 286-294. DOI: 10.1016/j.tplants.2009.02.003  [details] 
  • Slootweg, E., Tameling, W., Roosien, J., Lukasik, E., Joosten, M., Takken, F., ... Goverse, A. (2009). An outlook on the localisation and structure-function relationships of R proteins in Solanum. Potato Research, 52(3), 229-235. DOI: 10.1007/s11540-009-9130-9  [details] 

2008

  • Tameling, W. I. L., & Takken, F. L. W. (2008). Resistance proteins: Scouts of the plant innate immune system. European Journal of Plant Pathology, 121(3), 243-255. DOI: 10.1007/s10658-007-9187-8  [details] 
  • van Ooijen, G., Mayr, G., Albrecht, M., Cornelissen, B. J. C., & Takken, F. L. W. (2008). Transcomplementation, but not physical association of the CC-NB-ARC and LRR domains of tomato R protein Mi-1.2 is altered by mutations in the ARC2 subdomain. Molecular Plant, 1(3), 401-410. DOI: 10.1093/mp/ssn009  [details] 
  • van Ooijen, G., Mayr, G., Kasiem, M. M. A., Albrecht, M., Cornelissen, B. J. C., & Takken, F. L. W. (2008). Structure-function analysis of the NB-ARC domain of plant disease resistance proteins. Journal of Experimental Botany, 59(6), 1383-1397. DOI: 10.1093/jxb/ern045  [details] 
  • van den Burg, H. A., Tsitsigiannis, D. I., Rowland, O., Lo, J., Rallapalli, G., MacLean, D., ... Jones, J. D. G. (2008). The F-box protein ACRE189/ACIF1 regulates cell death and defense responses activated during pathogen recognition in tobacco and tomato. The Plant Cell, 20(3), 697-719. DOI: 10.1105/tpc.107.056978  [details] 

2007

  • van Ooijen, G., van den Burg, H. A., Cornelissen, B. J. C., & Takken, F. L. W. (2007). Structure and function of Resistance proteins in solanaceous plants. Annual Review of Phytopathology, 45, 43-72. DOI: 10.1146/annurev.phyto.45.062806.094430  [details] 
  • Gabriels, S. H. E. J., Vossen, J. H., Ekengren, S. K., van Ooijen, G., Abd-El-Haliem, A. M., van den Berg, G. C. M., ... Joosten, M. H. A. J. (2007). An NB-LRR protein required for HR signalling mediated by both extra- and intracellular resistance proteins. Plant Journal, 50(1), 14-28. DOI: 10.1111/j.1365-313X.2007.03027.x  [details] 

2006

  • Takken, F. L. W., Albrecht, M., & Tameling, W. I. L. (2006). Resistance proteins: molecular switches of plant defence. Current Opinion in Plant Biology, 9, 383-390. DOI: 10.1016/j.pbi.2006.05.009  [details] 
  • Takken, F. L. W., Tameling, W. I. L., Vossen, J. H., Albrecht, M., Berden, J. A., van Ooijen, G., & Cornelissen, B. J. C. (2006). The NB-ARC domain of R proteins acts as a molecular switch regulating plant innate immunity. In Q. C. Lópes-Lara Sánchez, S, & Geiger, O. (Eds.), Biology of Plant-Microbe Interactions. (pp. 225-230) [details] 
  • Tameling, W. I. L., Vossen, J. H., Albrecht, M., Lengauer, T., Berden, J. A., Haring, M. A., ... Takken, F. L. W. (2006). Mutations in the NB-ARC domain of I-2 that impair ATP hydrolysis cause autoactivation. Plant Physiology, 140, 1233-1245. DOI: 10.1104/pp.105.073510  [details] 
  • Albrecht, M., & Takken, F. L. W. (2006). Update on the domain architectures of NLRs and R proteins. Biochemical and Biophysical Research Communications, 339(2), 459-462. DOI: 10.1016/j.bbrc.2005.10.074  [details] 
  • Gabriels, S. H. E. J., Takken, F. L. W., Vossen, J. H., de Jong, C. F., Liu, Q., Turk, S. C. H. J., ... Joosten, M. H. A. J. (2006). cDNA-AFLP combined with functional analysis reveals novel genes involved in the hypersensitive response. Molecular Plant-Microbe Interactions, 19, 567-576. DOI: 10.1094/MPMI-19-0567  [details] 

2005

  • de la Fuente van Bentem, S., Vossen, J. H., de Vries, K. J., van Wees, A. C. M., Tameling, W. I. L., Dekker, H. L., ... Cornelissen, B. J. C. (2005). Heat shock protein 90 and its co-chaperone protein phosphatase 5 interact with distinct regions of the tomato I-2 disease resistance protein. Plant Journal, 43, 284-298. DOI: 10.1111/j.1365-313X.2005.02450.x  [details] 

2004

  • Takken, F. L. W., van Wijk, R., Michielse, C. B., Houterman, P. M., Ram, A. F., & Cornelissen, B. J. C. (2004). A one-step method to convert vectors into binary vectors suited for Agrobacterium-mediated transformation. Current Genetics, 45, 242-248. DOI: 10.1007/s00294-003-0481-5  [details] 

2002

  • Tameling, W. I. L., Elzinga, S. D. J., Darmin, P. S., Vossen, J. H., Takken, F. L. W., Haring, M. A., & Cornelissen, B. J. C. (2002). The tomato R gene products I-2 and Mi-1 are functional ATP binding proteins with ATPase activity. The Plant Cell, 14, 2929-2939. DOI: 10.1105/tpc.005793  [details] 
  • Luderer, R., Takken, F. L. W., de Wit, P. J. G. M., & Joosten, M. H. A. J. (2002). Cladosporium fulvum overcomes Cf-2-mediated resistance by producing truncated AVR2 elicitor proteins. Molecular Microbiology, 45(3), 875-884. DOI: 10.1046/j.1365-2958.2002.03060.x  [details] 
  • de Jong, C. F., Takken, F. L. W., Cai, X., de Wit, P. J. G. M., & Joosten, M. H. A. J. (2002). Attenuation of Cf-mediated defense responses at elevated temperatures correlates with a decrease in elicitor-binding sites. Molecular Plant-Microbe Interactions, 15(10), 1040-1049. DOI: 10.1094/MPMI.2002.15.10.1040  [details] 
  • de Wit, P. J. G. M., Brandwagt, B. F., van den Burg, H. A., Cai, X., van der Hoorn, R. A. L., de Jong, C. F., ... Joosten, M. H. A. J. (2002). The molecular basis of co-evolution between Cladosporium fulvum and tomato. Antonie van Leeuwenhoek, 81, 409-412. DOI: 10.1023/A:1020553120889  [details] 

2010

  • van den Burg, H. A., & Takken, F. L. W. (2010). SUMO-, MAPK-, and resistance protein-signaling converge at transcription complexes that regulate plant innate immunity. Plant Signaling & Behavior, 5(12), 1587-1591. DOI: 10.4161/psb.5.12.13913  [details] 

2008

  • Tameling, W. I. L., & Takken, F. L. W. (2008). Resistance proteins: Scouts of the plant innate immune system. In D. B. Collinge, L. Munk, & B. M. Cooke (Eds.), Sustainable disease management in a European context (pp. 243-255). Springer. DOI: 10.1007/978-1-4020-8780-6_4  [details] 

2005

  • Takken, F. L. W. (2005). Overeenkomsten en verschillen in ziekteresistentie tussen plant en dier. Gewasbescherming, 36, 262. [details] 

2004

  • Joosten, M. H. A. J., de Jong, C. F., de Wit, P. J. G. M., Takken, F. L. W., & Turk, S. C. H. J. (2004). Nucleotide sequences involved in plant resistance.

2003

  • Turk, S. C. H. J., Takken, F. L. W., de Jong, C. F., Joosten, M. H. A. J., & de Wit, P. J. G. M. (2003). Nucleotide sequences involved in plant disease resistance

2002

  • Takken, F. L. W., & de Wit, P. J. G. M. (2002). Elicitor from Cladosporium

Talk / presentation

  • Takken, F.L.W. (speaker) (19-5-2011): The role of pathogen effectors in NLR-mediated plant innate immunity, International Meeting of the Collaborative Research Center SFB 648 “ Communication in Plants and their Responses to the Environment”, Halle (Saale), Germany.
  • Takken, F.L.W. (speaker) (8-10-2010): Molecular co-evolution in the tomato-Fusarium pathosystem, Justus Liebig University , Giessen, Germany, Giessen, Germany.
  • Takken, F.L.W. (invited speaker) (8-6-2010): Functional analysis of Fusarium oxysporum f.sp. lycopersici effector proteins., DFG funded colloquium: 'Microbial reprogramming of plant cell development' priority project SPP1212 'Plant-Micro', Freising, Germany.
  • Takken, F.L.W. (speaker) (11-5-2010): The arms race between effectors of Fusarium oxysporum and resistance proteins of tomato., Seminar series School of Biological and Biomedical Sciences, Durham University,, Durham, UK.
  • Takken, F.L.W. (speaker) (1-8-2009): How to resist a resistance protein?, British Society for Plant Pathology, Presidential Meeting 2009, Oxford, UK.
  • Takken, F.L.W. (speaker) (19-7-2009): How to resist tomato resistance proteins?, XIV International Congress on Molecular Plant-MicrobeInteractions, Quebec, Canada.
  • Takken, F.L.W. (speaker) (22-9-2008): Structure-function analysis of plant NB-LRR disease resistance proteins at the SFB 670, Structure Function and Evolution of Innate immunity, Max-Planck-Institut für Züchtungsforschung Koln, Germany.
  • Takken, F.L.W. (speaker) (9-9-2008): Resistance proteins: scouts of the plant innate immune system, 18th EUCARPIA congress, "Modern Variety Breeding for Present and Future Needs, Valencia, Spain.
  • Takken, F.L.W. (speaker) (25-6-2008): Resistance proteins: scouts of the plant innate immune system., 4th EPSO Conference, Presqu'ile de Giens (Côte d'Azur), France.
  • Takken, F.L.W. (speaker) (18-6-2008): Resistance proteins: scouts of the plant innate immune system., EPS/Bioexploit Summerschool “On the evolution of plant-pathogen interactions: from principles to practices, Wageningen, the Netherlands.
  • Takken, F.L.W. (speaker) (1-5-2008): Molecular aspects of I-2 mediated resistance to Fusarium oxysporum., Max-Planck-Institut für Züchtungsforschung, Koln, Germany.
  • Takken, F.L.W. (speaker) (10-2-2008): Function of the Nucleotide Binding Domain for R Proteins, Keystone Symposium on Plant Innate Immunity, Resort in Keystone, Colorado, USA.
  • Takken, F.L.W. (speaker) (7-12-2007): Molecular mechanisms regulating the activity of plant NB-LRR resistance proteins., seminar: Institute of Plant Biology., Zurich, Switzerland.
  • Takken, F.L.W. (speaker) (20-11-2007): The NB-ARC domain of R proteins acts as a molecular switch regulating plant innate immunity., seminar: Max von Pettenkofer Institut., LMU München, Germany.
  • Lukasik, E. (speaker), Cornelissen, B.J.C. (speaker) & Takken, F.L.W. (speaker) (17-10-2007): Role of nucleotide binding for R protein function., Bioexploit Meeting., Lunteren, the Netherlands.
  • Takken, F.L.W. (speaker) (12-7-2007): Host-Pathogen Interaction from Plants to Mammals -Distinct and Shared Pathways of Immune Defence., seminar: Inflammatory diseases of barrier organs., University Hospital, Kiel, Germany.
  • van Ooijen, G. (speaker), Cornelissen, B.J.C. (speaker) & Takken, F.L.W. (speaker) (1-1-2007): A role for the conserved MHD motif for R protein function., XIIIth International Congress on Molecular Plant-Microbe Interactions., Sorrento, Italy.
  • Takken, F.L.W. (speaker) (27-11-2006): The NB-ARC domain: an NTP-hydrolysing molecular switch., Comparative Biology of Innate Immune Systems, New York, USA.
  • Takken, F.L.W. (speaker), Tameling, W.I.L. (speaker), Albrecht, M. (speaker), van Ooijen, G. (speaker), de Vroomen, M.J. (speaker), de Vries, K.J. (speaker) & Cornelissen, B.J.C. (speaker) (13-8-2006): R proteins, molecular switches of plant defence., 8th Conference of the European Foundation for Plant Pathology & British Society of Plant Pathology Presedential Meeting, Copenhagen, Denmark.
  • Takken, F.L.W. (speaker) (2-2-2006): Innate immune receptors in plants and animals; do anlogous modules have comparable functions?, EPS Symposium Intracellular signalling, UvA, Amsterdam, The Netherlands.
  • Takken, F.L.W. (speaker), Tameling, W.I.L. (speaker), Vossen, J.H. (speaker), Albrecht, M. (speaker), Berden, J.A. (speaker), Haring, M.A. (speaker) & Cornelissen, B.J.C. (speaker) (14-12-2005): The NB-ARC domain of R proteins acts as a molecular switch regulating plant innate immunity., XII Int. congress on Molecular-Plant Microbe Interactions., Merida, Mexico.
  • Takken, F.L.W. (speaker) (27-4-2005): Overeenkomsten en verschillen in ziekteresistentie tussen plant en dier., Meeting: In gewasbeschermingsmanifestatie: Is het al tijd om te oogsten., Ede, The Netherlands.
  • Takken, F.L.W. (speaker) (27-4-2005): R proteins: molecular switches of disease resistance signalling., In 4e Gewasbeschermingsmanifestatie, Ede , The Netherlands.
  • Takken, F.L.W. (speaker) (4-4-2005): The role of nucleotide binding for R protein function., EPS Symposium, Lunteren, The Netherlands.
  • Takken, F.L.W. (speaker) (26-7-2004): R proteins: Molecular switches of disease resistance signalling., Invitation from the Boyce Thompson Institute at the Cornell University, Ithaca, United States.
  • Takken, F.L.W. (speaker) (6-4-2004): R proteins: molecular switches of disease resistance signalling., ALW/EPW Meeting, Lunteren, Nederland.
  • Takken, F.L.W. (speaker) (11-3-2004): R proteins: molecular switches of disease resistance signalling., 10th Nederland Biotechnology Conference, Ede, Nederland.
  • Takken, F.L.W. (speaker) (10-4-2003): Elucidation of the molecular and biochemical basis of I-2 mediated Fusarium resistance, Keystone Plant Biology: Functions and control of cell death - symposium, Snowbird, Utah, VS.
  • Takken, F.L.W. (speaker) (30-1-2003): The molecular and biochemical basis of I-2 mediated Fusarium resistance in tomato, WCS dat, Utrecht.
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